Practical course	RNA EXPRESSION ANALYSIS USING DNA MICROARRAYS	Biochemistry and Fundamental Medicine
Date	Nov 10-14, 2008
Organisers	Lausanne DNA Array Facility, Center for Integrative Genomics, University of Lausanne
Site	Center for Integrative Genomic
Theme	The development and widespread use of high-density DNA microarrays have been important responses to the explosion of sequence information produced by genome science. Principal among the application of microarrays is the large-scale analysis of gene expression -- often referred to as expression profiling. The power of this application lies in its ability to determine the expression patterns of thousands of genes in a single experiment. In this course, students will receive “hands-on” training in the basic steps of a microarray expression profiling experiment. This will include: 1. Isolation of total RNA 2. Qualitative and quantitative analysis of total RNA 3. Microarray hybridization target synthesis, purification and quality assessment 4. Microarray hybridization and washing, scanning and image analysis 5. Data quality assessment, normalization and determination of expression values 6. Statistical analysis of microarray data Additional topics that will be covered in lectures or through demonstrations include: 1. Statistical and practical aspects of experimental design 2. High-level statistical analysis of data 3. Data mining – e.g. pathway analysis of microarray results
Requirements	Please send a short CV and letter of motivation by mail or email to: Keith.Harshman@unil.ch Lausanne DNA array Facility Génopode-UNIL 1015 Lausanne
Deadline	Oct 01, 2008
Comments		Go to registration

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